Mechanism of lignin degradation via white rot fungi explored using spectral analysis and gas chromatography-mass spectrometry

The mechanism of lignin degradation via white rot fungi was studied. Phanerochaete chrysosporium and Pleurotus ostreatus were used for all the experiments, i.e., measuring concentration structure alkali lignins studying effect substrate enzyme activity on removal. Gas chromatography-mass spectrometry performed reaction liquid system. Alkali had a characteristic absorption spectrum with peak at approximately 280 nm. Precipitation in laccase (Lac) system occurred earlier, as well being more obvious than that manganese peroxidase (Mnp) maximum removal 29.4% Mnp 40 mg/L. increased concentration-dependent manner Lac increase led to an control group significantly lower experimental systems. mainly produced organic acids, esters, aromatic substances. In conclusion, could effectively remove lignin, which precipitation played major role, followed by enzymolysis; enzymolysis associated activity.